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Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts

Duss O, Stepanyuk GA, Grot A, O’Leary SE, Puglisi JD, Williamson JR. Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts. Nat Commun. 2018 Nov 30;9(1):5087. doi: 10.1038/s41467-018-07423-3. PMID: 30504830.
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De novo computational RNA modeling into cryo-EM maps of large ribonucleoprotein complexes

Kappel K, Liu S, Larsen KP, Skiniotis G, Puglisi EV, Puglisi JD, Zhou ZH, Zhao R, Das R. De novo computational RNA modeling into cryo-EM maps of large ribonucleoprotein complexes. Nat Methods. 2018 Nov;15(11):947-954. doi: 10.1038/s41592-018-0172-2. Epub 2018 Oct 30. […]
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Dynamic Interplay of RNA and Protein in the Human Immunodeficiency Virus-1 Reverse Transcription Initiation Complex

Coey AT, Larsen KP, Choi J, Barrero DJ, Puglisi JD, Puglisi EV. Dynamic Interplay of RNA and Protein in the Human Immunodeficiency Virus-1 Reverse Transcription Initiation Complex. J Mol Biol. 2018 Dec 7;430(24):5137-5150. doi: 10.1016/j.jmb.2018.08.029. Epub 2018 Sep 7. PMID: […]
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How Messenger RNA and Nascent Chain Sequences Regulate Translation Elongation

Choi J, Grosely R, Prabhakar A, Lapointe CP, Wang J, Puglisi JD. How Messenger RNA and Nascent Chain Sequences Regulate Translation Elongation. Annu Rev Biochem. 2018 Jun 20;87:421-449. doi: 10.1146/annurev-biochem-060815-014818. PMID: 29925264.
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Architecture of an HIV-1 reverse transcriptase initiation complex

Larsen KP, Mathiharan YK, Kappel K, Coey AT, Chen DH, Barrero D, Madigan L, Puglisi JD, Skiniotis G, Puglisi EV. Architecture of an HIV-1 reverse transcriptase initiation complex. Nature. 2018 May;557(7703):118-122. doi: 10.1038/s41586-018-0055-9. Epub 2018 Apr 25. PMID: 29695867.
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2′-O-methylation in mRNA disrupts tRNA decoding during translation elongation

Choi J, Indrisiunaite G, DeMirci H, Ieong KW, Wang J, Petrov A, Prabhakar A, Rechavi G, Dominissini D, He C, Ehrenberg M, Puglisi JD. 2′-O-methylation in mRNA disrupts tRNA decoding during translation elongation. Nat Struct Mol Biol. 2018 Mar;25(3):208-216. doi: […]
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Fluorescently-tagged human eIF3 for single-molecule spectroscopy

Johnson AG, Petrov AN, Fuchs G, Majzoub K, Grosely R, Choi J, Puglisi JD. Fluorescently-tagged human eIF3 for single-molecule spectroscopy. Nucleic Acids Res. 2018 Jan 25;46(2):e8. doi: 10.1093/nar/gkx1050. PMID: 29136179.
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