The mechanism of Eukaryotic Translation Initiation and Elongation.
Eukaryotic translation occurs by the same basic mechanism as in prokaryotic organisms. However, the processes of initiation and elongation are more complex and regulated. We are using a number of biophysical and structural approaches to characterized eukaryotic translation. These include:
1. Single-molecule fluorescence spectroscopy (with Prof. Steve Chu, Applied Physics, Stanford) to characterized the mechanism of translation initiation in yeast.
2. Single-molecule fluorescence analysis of the mechanism of translation elongation in yeast.
3. NMR structural studies of translation initiation factors, and proteins involved in cell signaling with translation as an endpoint (eg. PKR system).