2021

Lawson MR, Lessen LN, Wang J, Prabhakar A, Corsepius NC, Green R, Puglisi JD. Mechanisms that ensure speed and fidelity in eukaryotic translation termination. Science. 2021 Aug 20;373(6557):876-882. doi: 10.1126/science.abi7801. PMID: 34413231.

Ha B, Larsen KP, Zhang J, Fu Z, Montabana E, Jackson LN, Chen DH, Puglisi EV. High-resolution view of HIV-1 reverse transcriptase initiation complexes and inhibition by NNRTI drugs. Nat Commun. 2021 May 4;12(1):2500. doi: 10.1038/s41467-021-22628-9. PMID: 33947853.

Ieong KW, Indrisiunaite G, Prabhakar A, Puglisi JD, Ehrenberg M. N 6-Methyladenosines in mRNAs reduce the accuracy of codon reading by transfer RNAs and peptide release factors. Nucleic Acids Res. 2021 Mar 18;49(5):2684-2699. doi: 10.1093/nar/gkab033. PMID: 33561188.

Lapointe CP, Grosely R, Johnson AG, Wang J, Fernández IS, Puglisi JD. Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2017715118. doi: 10.1073/pnas.2017715118. PMID: 33479166.

2020

Wang J, Wang J, Shin BS, Kim JR, Dever TE, Puglisi JD, Fernández IS. Structural basis for the transition from translation initiation to elongation by an 80S-eIF5B complex. Nat Commun. 2020 Oct 6;11(1):5003. doi: 10.1038/s41467-020-18829-3. PMID: 33024099.

Krupkin M, Jackson LN, Ha B, Puglisi EV. Advances in understanding the initiation of HIV-1 reverse transcription. Curr Opin Struct Biol. 2020 Dec;65:175-183. doi: 10.1016/j.sbi.2020.07.005. Epub 2020 Sep 8. PMID: 32916568.

Larsen KP, Choi J, Jackson LN, Kappel K, Zhang J, Ha B, Chen DH, Puglisi EV. Distinct Conformational States Underlie Pausing during Initiation of HIV-1 Reverse Transcription. J Mol Biol. 2020 Jul 24;432(16):4499-4522. doi: 10.1016/j.jmb.2020.06.003. Epub 2020 Jun 6. PMID: 32512005.

Johnson AG, Flynn RA, Lapointe CP, Ooi YS, Zhao ML, Richards CM, Qiao W, Yamada SB, Couthouis J, Gitler AD, Carette JE, Puglisi JD. A memory of eS25 loss drives resistance phenotypes. Nucleic Acids Res. 2020 Jul 27;48(13):7279-7297. doi: 10.1093/nar/gkaa444. PMID: 32463448.

O’Loughlin S, Capece MC, Klimova M, Wills NM, Coakley A, Samatova E, O’Connor PBF, Loughran G, Weissman JS, Baranov PV, Rodnina MV, Puglisi JD, Atkins JF. Polysomes Bypass a 50-Nucleotide Coding Gap Less Efficiently Than Monosomes Due to Attenuation of a 5′ mRNA Stem-Loop and Enhanced Drop-off. J Mol Biol. 2020 Jul 24;432(16):4369-4387. doi: 10.1016/j.jmb.2020.05.010. Epub 2020 May 23. PMID: 32454154.

Choi J, O’Loughlin S, Atkins JF, Puglisi JD. The energy landscape of -1 ribosomal frameshifting. Sci Adv. 2020 Jan 1;6(1):eaax6969. doi: 10.1126/sciadv.aax6969. eCollection 2020 Jan. PMID: 31911945.

2019

Verma M, Choi J, Cottrell KA, Lavagnino Z, Thomas EN, Pavlovic-Djuranovic S, Szczesny P, Piston DW, Zaher HS, Puglisi JD, Djuranovic S. A short translational ramp determines the efficiency of protein synthesis. Nat Commun. 2019 Dec 18;10(1):5774. doi: 10.1038/s41467-019-13810-1. PMID: 31852903.

Choi J, Marks J, Zhang J, Chen DH, Wang J, Vázquez-Laslop N, Mankin AS, Puglisi JD. Dynamics of the context-specific translation arrest by chloramphenicol and linezolid. Nat Chem Biol. 2020 Mar;16(3):310-317. doi: 10.1038/s41589-019-0423-2. Epub 2019 Dec 16. PMID: 31844301.

Chandrasekaran V, Juszkiewicz S, Choi J, Puglisi JD, Brown A, Shao S, Ramakrishnan V, Hegde RS. Mechanism of ribosome stalling during translation of a poly(A) tail. Nat Struct Mol Biol. 2019 Dec;26(12):1132-1140. doi: 10.1038/s41594-019-0331-x. Epub 2019 Nov 25. PMID: 31768042.

Duss O, Stepanyuk GA, Puglisi JD, Williamson JR. Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA Folding. Cell. 2019 Nov 27;179(6):1357-1369.e16. doi: 10.1016/j.cell.2019.10.035. Epub 2019 Nov 21. PMID: 31761533.

Wang J, Johnson AG, Lapointe CP, Choi J, Prabhakar A, Chen DH, Petrov AN, Puglisi JD. eIF5B gates the transition from translation initiation to elongation. Nature. 2019 Sep;573(7775):605-608. doi: 10.1038/s41586-019-1561-0. Epub 2019 Sep 18. PMID: 31534220.

Yamada SB, Gendron TF, Niccoli T, Genuth NR, Grosely R, Shi Y, Glaria I, Kramer NJ, Nakayama L, Fang S, Dinger TJI, Thoeng A, Rocha G, Barna M, Puglisi JD, Partridge L, Ichida JK, Isaacs AM, Petrucelli L, Gitler AD. RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats. Nat Neurosci. 2019 Sep;22(9):1383-1388. doi: 10.1038/s41593-019-0455-7. Epub 2019 Jul 29. PMID: 31358992.

Larsen KP, Choi J, Prabhakar A, Puglisi EV, Puglisi JD. Relating Structure and Dynamics in RNA Biology. Cold Spring Harb Perspect Biol. 2019 Jul 1;11(7):a032474. doi: 10.1101/cshperspect.a032474. PMID: 31262948.

Choi J, Grosely R, Puglisi EV, Puglisi JD. Expanding single-molecule fluorescence spectroscopy to capture complexity in biology. Curr Opin Struct Biol. 2019 Oct;58:233-240. doi: 10.1016/j.sbi.2019.05.005. Epub 2019 Jun 15. PMID: 31213390.

Johnson AG, Lapointe CP, Wang J, Corsepius NC, Choi J, Fuchs G, Puglisi JD. RACK1 on and off the ribosome. RNA. 2019 Jul;25(7):881-895. doi: 10.1261/rna.071217.119. Epub 2019 Apr 25. PMID: 31023766.

2018

Duss O, Stepanyuk GA, Grot A, O’Leary SE, Puglisi JD, Williamson JR. Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts. Nat Commun. 2018 Nov 30;9(1):5087. doi: 10.1038/s41467-018-07423-3. PMID: 30504830.

Kappel K, Liu S, Larsen KP, Skiniotis G, Puglisi EV, Puglisi JD, Zhou ZH, Zhao R, Das R. De novo computational RNA modeling into cryo-EM maps of large ribonucleoprotein complexes. Nat Methods. 2018 Nov;15(11):947-954. doi: 10.1038/s41592-018-0172-2. Epub 2018 Oct 30. PMID: 30377372.

Coey AT, Larsen KP, Choi J, Barrero DJ, Puglisi JD, Puglisi EV. Dynamic Interplay of RNA and Protein in the Human Immunodeficiency Virus-1 Reverse Transcription Initiation Complex. J Mol Biol. 2018 Dec 7;430(24):5137-5150. doi: 10.1016/j.jmb.2018.08.029. Epub 2018 Sep 7. PMID: 30201267.

Choi J, Grosely R, Prabhakar A, Lapointe CP, Wang J, Puglisi JD. How Messenger RNA and Nascent Chain Sequences Regulate Translation Elongation. Annu Rev Biochem. 2018 Jun 20;87:421-449. doi: 10.1146/annurev-biochem-060815-014818. PMID: 29925264.

Prabhakar A, Puglisi EV, Puglisi JD. Single-Molecule Fluorescence Applied to Translation. Cold Spring Harb Perspect Biol. 2019 Jan 2;11(1):a032714. doi: 10.1101/cshperspect.a032714. PMID: 29891562.

Larsen KP, Mathiharan YK, Kappel K, Coey AT, Chen DH, Barrero D, Madigan L, Puglisi JD, Skiniotis G, Puglisi EV. Architecture of an HIV-1 reverse transcriptase initiation complex. Nature. 2018 May;557(7703):118-122. doi: 10.1038/s41586-018-0055-9. Epub 2018 Apr 25. PMID: 29695867.

Choi J, Indrisiunaite G, DeMirci H, Ieong KW, Wang J, Petrov A, Prabhakar A, Rechavi G, Dominissini D, He C, Ehrenberg M, Puglisi JD. 2′-O-methylation in mRNA disrupts tRNA decoding during translation elongation. Nat Struct Mol Biol. 2018 Mar;25(3):208-216. doi: 10.1038/s41594-018-0030-z. Epub 2018 Feb 19. PMID: 29459784.

2017

Choi J, Puglisi JD. Three tRNAs on the ribosome slow translation elongation. Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):13691-13696. doi: 10.1073/pnas.1719592115. Epub 2017 Dec 11. PMID: 29229848.

Johnson AG, Petrov AN, Fuchs G, Majzoub K, Grosely R, Choi J, Puglisi JD. Fluorescently-tagged human eIF3 for single-molecule spectroscopy. Nucleic Acids Res. 2018 Jan 25;46(2):e8. doi: 10.1093/nar/gkx1050. PMID: 29136179.

Prabhakar A, Capece MC, Petrov A, Choi J, Puglisi JD. Post-termination Ribosome Intermediate Acts as the Gateway to Ribosome Recycling. Cell Rep. 2017 Jul 5;20(1):161-172. doi: 10.1016/j.celrep.2017.06.028. PMID: 28683310.

Prabhakar A, Choi J, Wang J, Petrov A, Puglisi JD. Dynamic basis of fidelity and speed in translation: Coordinated multistep mechanisms of elongation and termination. Protein Sci. 2017 Jul;26(7):1352-1362. doi: 10.1002/pro.3190. Epub 2017 May 23. PMID: 28480640.

Johnson AG, Grosely R, Petrov AN, Puglisi JD. Dynamics of IRES-mediated translation. Philos Trans R Soc Lond B Biol Sci. 2017 Mar 19;372(1716):20160177. doi: 10.1098/rstb.2016.0177. PMID: 28138065.

2016

Chen J, Choi J, O’Leary SE, Prabhakar A, Petrov A, Grosely R, Puglisi EV, Puglisi JD. The molecular choreography of protein synthesis: translational control, regulation, and pathways. Q Rev Biophys. 2016 Jan;49:e11. doi: 10.1017/S0033583516000056. Epub 2016 Jun 24. PMID: 27658712.

Coey A, Larsen K, Puglisi JD, Viani Puglisi E. Heterogeneous structures formed by conserved RNA sequences within the HIV reverse transcription initiation site. RNA. 2016 Nov;22(11):1689-1698. doi: 10.1261/rna.056804.116. Epub 2016 Sep 9. PMID: 27613581.

Navon SP, Kornberg G, Chen J, Schwartzman T, Tsai A, Puglisi EV, Puglisi JD, Adir N. Amino acid sequence repertoire of the bacterial proteome and the occurrence of untranslatable sequences. Proc Natl Acad Sci U S A. 2016 Jun 28;113(26):7166-70. doi: 10.1073/pnas.1606518113. Epub 2016 Jun 15. PMID: 27307442.

Petrov A, Grosely R, Chen J, O’Leary SE, Puglisi JD. Multiple Parallel Pathways of Translation Initiation on the CrPV IRES. Mol Cell. 2016 Apr 7;62(1):92-103. doi: 10.1016/j.molcel.2016.03.020. PMID: 27058789.

Tsai A, Puglisi JD, Uemura S. Probing the Translation Dynamics of Ribosomes Using Zero-Mode Waveguides. Prog Mol Biol Transl Sci. 2016;139:1-43. doi: 10.1016/bs.pmbts.2015.10.006. Epub 2015 Nov 2. PMID: 26970189.

Choi J, Ieong KW, Demirci H, Chen J, Petrov A, Prabhakar A, O’Leary SE, Dominissini D, Rechavi G, Soltis SM, Ehrenberg M, Puglisi JD. N(6)-methyladenosine in mRNA disrupts tRNA selection and translation-elongation dynamics. Nat Struct Mol Biol. 2016 Feb;23(2):110-5. doi: 10.1038/nsmb.3148. Epub 2016 Jan 11. PMID: 26751643.

2015

Sierra RG, Gati C, Laksmono H, Dao EH, Gul S, Fuller F, Kern J, Chatterjee R, Ibrahim M, Brewster AS, Young ID, Michels-Clark T, Aquila A, Liang M, Hunter MS, Koglin JE, Boutet S, Junco EA, Hayes B, Bogan MJ, Hampton CY, Puglisi EV, Sauter NK, Stan CA, Zouni A, Yano J, Yachandra VK, Soltis SM, Puglisi JD, DeMirci H. Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II. Nat Methods. 2016 Jan;13(1):59-62. doi: 10.1038/nmeth.3667. Epub 2015 Nov 30. PMID: 26619013.

Siprashvili Z, Webster DE, Johnston D, Shenoy RM, Ungewickell AJ, Bhaduri A, Flockhart R, Zarnegar BJ, Che Y, Meschi F, Puglisi JD, Khavari PA. The noncoding RNAs SNORD50A and SNORD50B bind K-Ras and are recurrently deleted in human cancer. Nat Genet. 2016 Jan;48(1):53-8. doi: 10.1038/ng.3452. Epub 2015 Nov 23. PMID: 26595770.

Chen J, Coakley A, O’Connor M, Petrov A, O’Leary SE, Atkins JF, Puglisi JD. Coupling of mRNA Structure Rearrangement to Ribosome Movement during Bypassing of Non-coding Regions. Cell. 2015 Nov 19;163(5):1267-1280. doi: 10.1016/j.cell.2015.10.064. PMID: 26590426.

Nilsson OB, Hedman R, Marino J, Wickles S, Bischoff L, Johansson M, Müller-Lucks A, Trovato F, Puglisi JD, O’Brien EP, Beckmann R, von Heijne G. Cotranslational Protein Folding inside the Ribosome Exit Tunnel. Cell Rep. 2015 Sep 8;12(10):1533-40. doi: 10.1016/j.celrep.2015.07.065. Epub 2015 Aug 28. PMID: 26321634.

Puglisi JD. Synthetic biology: Ribosomal ties that bind. Nature. 2015 Aug 6;524(7563):45-6. doi: 10.1038/nature14642. Epub 2015 Jul 29. PMID: 26222027.

Puglisi JD. Protein synthesis. The delicate dance of translation and folding. Science. 2015 Apr 24;348(6233):399-400. doi: 10.1126/science.aab2157. PMID: 25908811.

Puglisi JD. RNA dances to center stage. RNA. 2015 Apr;21(4):712-3. doi: 10.1261/rna.051078.115. PMID: 25780204.

2014

Capece MC, Kornberg GL, Petrov A, Puglisi JD. A simple real-time assay for in vitro translation. RNA. 2015 Feb;21(2):296-305. doi: 10.1261/rna.047159.114. Epub 2014 Dec 18. PMID: 25525154.

Fuchs G, Petrov AN, Marceau CD, Popov LM, Chen J, O’Leary SE, Wang R, Carette JE, Sarnow P, Puglisi JD. Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site. Proc Natl Acad Sci U S A. 2015 Jan 13;112(2):319-25. doi: 10.1073/pnas.1421328111. Epub 2014 Dec 16. PMID: 25516984.

Noriega TR, Chen J, Walter P, Puglisi JD. Real-time observation of signal recognition particle binding to actively translating ribosomes. Elife. 2014 Oct 30;3:e04418. doi: 10.7554/eLife.04418. PMID: 25358118.

Chen J, Petrov A, Johansson M, Tsai A, O’Leary SE, Puglisi JD. Dynamic pathways of -1 translational frameshifting. Nature. 2014 Aug 21;512(7514):328-32. doi: 10.1038/nature13428. Epub 2014 Jun 11. PMID: 24919156.

Johansson M, Chen J, Tsai A, Kornberg G, Puglisi JD. Sequence-dependent elongation dynamics on macrolide-bound ribosomes. Cell Rep. 2014 Jun 12;7(5):1534-1546. doi: 10.1016/j.celrep.2014.04.034. Epub 2014 May 15. PMID: 24836000.

Tsai A, Kornberg G, Johansson M, Chen J, Puglisi JD. The dynamics of SecM-induced translational stalling. Cell Rep. 2014 Jun 12;7(5):1521-1533. doi: 10.1016/j.celrep.2014.04.033. Epub 2014 May 15. PMID: 24836001.

Noriega TR, Tsai A, Elvekrog MM, Petrov A, Neher SB, Chen J, Bradshaw N, Puglisi JD, Walter P. Signal recognition particle-ribosome binding is sensitive to nascent chain length. J Biol Chem. 2014 Jul 11;289(28):19294-305. doi: 10.1074/jbc.M114.563239. Epub 2014 May 7. PMID: 24808175.

Chen J, Dalal RV, Petrov AN, Tsai A, O’Leary SE, Chapin K, Cheng J, Ewan M, Hsiung PL, Lundquist P, Turner SW, Hsu DR, Puglisi JD. High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence. Proc Natl Acad Sci U S A. 2014 Jan 14;111(2):664-9. doi: 10.1073/pnas.1315735111. Epub 2013 Dec 30. PMID: 24379388.

2013

O’Leary SE, Petrov A, Chen J, Puglisi JD. Dynamic recognition of the mRNA cap by Saccharomyces cerevisiae eIF4E. Structure. 2013 Dec 3;21(12):2197-207. doi: 10.1016/j.str.2013.09.016. Epub 2013 Oct 31. PMID: 24183571.

Petrov A, Tsa A, Puglisi JD. Analysis of RNA by analytical polyacrylamide gel electrophoresis. Methods Enzymol. 2013;530:301-13. doi: 10.1016/B978-0-12-420037-1.00016-6. PMID: 24034328.

Petrov A, Wu T, Puglisi EV, Puglisi JD. RNA purification by preparative polyacrylamide gel electrophoresis. Methods Enzymol. 2013;530:315-30. doi: 10.1016/B978-0-12-420037-1.00017-8. PMID: 24034329.

Simonetti A, Marzi S, Billas IM, Tsai A, Fabbretti A, Myasnikov AG, Roblin P, Vaiana AC, Hazemann I, Eiler D, Steitz TA, Puglisi JD, Gualerzi CO, Klaholz BP. Involvement of protein IF2 N domain in ribosomal subunit joining revealed from architecture and function of the full-length initiation factor. Proc Natl Acad Sci U S A. 2013 Sep 24;110(39):15656-61. doi: 10.1073/pnas.1309578110. Epub 2013 Sep 12. PMID: 24029017.

Chen J, Petrov A, Tsai A, O’Leary SE, Puglisi JD. Coordinated conformational and compositional dynamics drive ribosome translocation. Nat Struct Mol Biol. 2013 Jun;20(6):718-27. doi: 10.1038/nsmb.2567. Epub 2013 Apr 28. PMID: 23624862.

Tsai A, Uemura S, Johansson M, Puglisi EV, Marshall RA, Aitken CE, Korlach J, Ehrenberg M, Puglisi JD. The impact of aminoglycosides on the dynamics of translation elongation. Cell Rep. 2013 Feb 21;3(2):497-508. doi: 10.1016/j.celrep.2013.01.027. Epub 2013 Feb 14. PMID: 23416053.

2012

Chen J, Tsai A, O’Leary SE, Petrov A, Puglisi JD. Unraveling the dynamics of ribosome translocation. Curr Opin Struct Biol. 2012 Dec;22(6):804-14. doi: 10.1016/j.sbi.2012.09.004. Epub 2012 Nov 8. PMID: 23142574.

Petrov A, Chen J, O’Leary S, Tsai A, Puglisi JD. Single-molecule analysis of translational dynamics. Cold Spring Harb Perspect Biol. 2012 Sep 1;4(9):a011551. doi: 10.1101/cshperspect.a011551. PMID: 22798542.

Harvey CJ, Puglisi JD, Pande VS, Cane DE, Khosla C. Precursor directed biosynthesis of an orthogonally functional erythromycin analogue: selectivity in the ribosome macrolide binding pocket. J Am Chem Soc. 2012 Jul 25;134(29):12259-65. doi: 10.1021/ja304682q. Epub 2012 Jul 11. PMID: 22741553.

Tsai A, Petrov A, Marshall RA, Korlach J, Uemura S, Puglisi JD. Heterogeneous pathways and timing of factor departure during translation initiation. Nature. 2012 Jul 19;487(7407):390-3. doi: 10.1038/nature11172. PMID: 22722848.

Puglisi JD, Williamson JR. Digging deep into nucleic acid structure and nucleic acid protein recognition. Curr Opin Struct Biol. 2012 Jun;22(3):249-50. doi: 10.1016/j.sbi.2012.05.003. Epub 2012 May 24. PMID: 22632873.

Chen J, Tsai A, Petrov A, Puglisi JD. Nonfluorescent quenchers to correlate single-molecule conformational and compositional dynamics. J Am Chem Soc. 2012 Apr 4;134(13):5734-7. doi: 10.1021/ja2119964. Epub 2012 Mar 23. PMID: 22428667.

Masuda T, Petrov AN, Iizuka R, Funatsu T, Puglisi JD, Uemura S. Initiation factor 2, tRNA, and 50S subunits cooperatively stabilize mRNAs on the ribosome during initiation. Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):4881-5. doi: 10.1073/pnas.1118452109. Epub 2012 Mar 12. PMID: 22411833.

2011

Puglisi EV, Puglisi JD. Secondary structure of the HIV reverse transcription initiation complex by NMR. J Mol Biol. 2011 Jul 29;410(5):863-74. doi: 10.1016/j.jmb.2011.04.024. PMID: 21763492.

Petrov A, Kornberg G, O’Leary S, Tsai A, Uemura S, Puglisi JD. Dynamics of the translational machinery. Curr Opin Struct Biol. 2011 Feb;21(1):137-45. doi: 10.1016/j.sbi.2010.11.007. Epub 2011 Jan 20. PMID: 21256733.

2010

Aitken CE, Puglisi JD. Following the intersubunit conformation of the ribosome during translation in real time. Nat Struct Mol Biol. 2010 Jul;17(7):793-800. doi: 10.1038/nsmb.1828. Epub 2010 Jun 20. PMID: 20562856.

Puglisi JD, Williamson JR. Nucleic acids continue to surprise. Curr Opin Struct Biol. 2010 Jun;20(3):259-61. doi: 10.1016/j.sbi.2010.04.005. Epub 2010 May 24. PMID: 20510604.

Petrov A, Puglisi JD. Site-specific labeling of Saccharomyces cerevisiae ribosomes for single-molecule manipulations. Nucleic Acids Res. 2010 Jul;38(13):e143. doi: 10.1093/nar/gkq390. Epub 2010 May 25. PMID: 20501598.

Uemura S, Aitken CE, Korlach J, Flusberg BA, Turner SW, Puglisi JD. Real-time tRNA transit on single translating ribosomes at codon resolution. Nature. 2010 Apr 15;464(7291):1012-7. doi: 10.1038/nature08925. PMID: 20393556.

Aitken CE, Petrov A, Puglisi JD. Single ribosome dynamics and the mechanism of translation. Annu Rev Biophys. 2010;39:491-513. doi: 10.1146/annurev.biophys.093008.131427. PMID: 20192783.

Bokoch MP, Zou Y, Rasmussen SG, Liu CW, Nygaard R, Rosenbaum DM, Fung JJ, Choi HJ, Thian FS, Kobilka TS, Puglisi JD, Weis WI, Pardo L, Prosser RS, Mueller L, Kobilka BK. Ligand-specific regulation of the extracellular surface of a G-protein-coupled receptor. Nature. 2010 Jan 7;463(7277):108-12. doi: 10.1038/nature08650. PMID: 20054398.

2009

Puglisi JD. Resolving the elegant architecture of the ribosome. Mol Cell. 2009 Dec 11;36(5):720-3. doi: 10.1016/j.molcel.2009.11.031. PMID: 20005832.

Elazar M, Liu M, McKenna SA, Liu P, Gehrig EA, Puglisi JD, Rossignol JF, Glenn JS. The anti-hepatitis C agent nitazoxanide induces phosphorylation of eukaryotic initiation factor 2alpha via protein kinase activated by double-stranded RNA activation. Gastroenterology. 2009 Nov;137(5):1827-35. doi: 10.1053/j.gastro.2009.07.056. Epub 2009 Aug 4. PMID: 19664635.

Marshall RA, Aitken CE, Puglisi JD. GTP hydrolysis by IF2 guides progression of the ribosome into elongation. Mol Cell. 2009 Jul 10;35(1):37-47. doi: 10.1016/j.molcel.2009.06.008. PMID: 19595714.

Shimoike T, McKenna SA, Lindhout DA, Puglisi JD. Translational insensitivity to potent activation of PKR by HCV IRES RNA. Antiviral Res. 2009 Sep;83(3):228-37. doi: 10.1016/j.antiviral.2009.05.004. Epub 2009 May 23. PMID: 19467267.

2008

Marshall RA, Dorywalska M, Puglisi JD. Irreversible chemical steps control intersubunit dynamics during translation. Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15364-9. doi: 10.1073/pnas.0805299105. Epub 2008 Sep 29. PMID: 18824686.

Puglisi JD, Doudna JA. Nucleic acids and their protein partners. Curr Opin Struct Biol. 2008 Jun;18(3):279-81. doi: 10.1016/j.sbi.2008.05.004. Epub 2008 Jun 9. PMID: 18547801.

Marshall RA, Aitken CE, Dorywalska M, Puglisi JD. Translation at the single-molecule level. Annu Rev Biochem. 2008;77:177-203. doi: 10.1146/annurev.biochem.77.070606.101431. PMID: 18518820.

Uemura S, Iizuka R, Ueno T, Shimizu Y, Taguchi H, Ueda T, Puglisi JD, Funatsu T. Single-molecule imaging of full protein synthesis by immobilized ribosomes. Nucleic Acids Res. 2008 Jul;36(12):e70. doi: 10.1093/nar/gkn338. Epub 2008 May 29. PMID: 18511463.

2007

Puglisi JD. Structural biology: the dance of domains. Nature. 2007 Dec 20;450(7173):1171-2. doi: 10.1038/4501171a. PMID: 18097392.

McKenna SA, Kim I, Puglisi EV, Lindhout DA, Aitken CE, Marshall RA, Puglisi JD. Purification and characterization of transcribed RNAs using gel filtration chromatography. Nat Protoc. 2007;2(12):3270-7. doi: 10.1038/nprot.2007.480. PMID: 18079727.

Puglisi EV, Puglisi JD. Probing the conformation of human tRNA(3)(Lys) in solution by NMR. FEBS Lett. 2007 Nov 13;581(27):5307-14. doi: 10.1016/j.febslet.2007.10.026. Epub 2007 Oct 24. PMID: 17963705.

Gonzalez RL Jr, Chu S, Puglisi JD. Thiostrepton inhibition of tRNA delivery to the ribosome. RNA. 2007 Dec;13(12):2091-7. doi: 10.1261/rna.499407. Epub 2007 Oct 19. PMID: 17951333.

Aitken CE, Marshall RA, Puglisi JD. An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments. Biophys J. 2008 Mar 1;94(5):1826-35. doi: 10.1529/biophysj.107.117689. Epub 2007 Oct 5. PMID: 17921203.

McKenna SA, Lindhout DA, Shimoike T, Puglisi JD. Biophysical and biochemical investigations of dsRNA-activated kinase PKR. Methods Enzymol. 2007;430:373-96. doi: 10.1016/S0076-6879(07)30014-1. PMID: 17913645.

Alekseyev VY, Liu CW, Cane DE, Puglisi JD, Khosla C. Solution structure and proposed domain domain recognition interface of an acyl carrier protein domain from a modular polyketide synthase. Protein Sci. 2007 Oct;16(10):2093-107. doi: 10.1110/ps.073011407. PMID: 17893358.

Lee TH, Blanchard SC, Kim HD, Puglisi JD, Chu S. The role of fluctuations in tRNA selection by the ribosome. Proc Natl Acad Sci U S A. 2007 Aug 21;104(34):13661-5. doi: 10.1073/pnas.0705988104. Epub 2007 Aug 15. PMID: 17699629.

Kim HD, Puglisi JD, Chu S. Fluctuations of transfer RNAs between classical and hybrid states. Biophys J. 2007 Nov 15;93(10):3575-82. doi: 10.1529/biophysj.107.109884. Epub 2007 Aug 10. PMID: 17693476.

Varani L, Bankovich AJ, Liu CW, Colf LA, Jones LL, Kranz DM, Puglisi JD, Garcia KC. Solution mapping of T cell receptor docking footprints on peptide-MHC. Proc Natl Acad Sci U S A. 2007 Aug 7;104(32):13080-5. doi: 10.1073/pnas.0703702104. Epub 2007 Aug 1. PMID: 17670943.

McKenna SA, Lindhout DA, Shimoike T, Aitken CE, Puglisi JD. Viral dsRNA inhibitors prevent self-association and autophosphorylation of PKR. J Mol Biol. 2007 Sep 7;372(1):103-13. doi: 10.1016/j.jmb.2007.06.028. Epub 2007 Jun 15. PMID: 17619024.

Uemura S, Dorywalska M, Lee TH, Kim HD, Puglisi JD, Chu S. Peptide bond formation destabilizes Shine-Dalgarno interaction on the ribosome. Nature. 2007 Mar 22;446(7134):454-7. doi: 10.1038/nature05625. PMID: 17377584.

McKenna SA, Lindhout DA, Kim I, Liu CW, Gelev VM, Wagner G, Puglisi JD. Molecular framework for the activation of RNA-dependent protein kinase. J Biol Chem. 2007 Apr 13;282(15):11474-86. doi: 10.1074/jbc.M700301200. Epub 2007 Feb 6. PMID: 17284445.

2006

Kim I, McKenna SA, Viani Puglisi E, Puglisi JD. Rapid purification of RNAs using fast performance liquid chromatography (FPLC). RNA. 2007 Feb;13(2):289-94. doi: 10.1261/rna.342607. Epub 2006 Dec 18. PMID: 17179067.

McKenna SA, Kim I, Liu CW, Puglisi JD. Uncoupling of RNA binding and PKR kinase activation by viral inhibitor RNAs. J Mol Biol. 2006 May 19;358(5):1270-85. doi: 10.1016/j.jmb.2006.03.003. Epub 2006 Mar 20. PMID: 16580685.

Kim I, Liu CW, Puglisi JD. Specific recognition of HIV TAR RNA by the dsRNA binding domains (dsRBD1-dsRBD2) of PKR. J Mol Biol. 2006 Apr 28;358(2):430-42. doi: 10.1016/j.jmb.2006.01.099. Epub 2006 Feb 13. PMID: 16516925.

2005

Underwood KA, Swartz JR, Puglisi JD. Quantitative polysome analysis identifies limitations in bacterial cell-free protein synthesis. Biotechnol Bioeng. 2005 Aug 20;91(4):425-35. doi: 10.1002/bit.20529. PMID: 15991235.

Lukavsky PJ, Puglisi JD. Structure determination of large biological RNAs. Methods Enzymol. 2005;394:399-416. doi: 10.1016/S0076-6879(05)94016-0. PMID: 15808230.

Dorywalska M, Blanchard SC, Gonzalez RL, Kim HD, Chu S, Puglisi JD. Site-specific labeling of the ribosome for single-molecule spectroscopy. Nucleic Acids Res. 2005 Jan 12;33(1):182-9. doi: 10.1093/nar/gki151. Print 2005. PMID: 15647501.

2004

Otto GA, Puglisi JD. The pathway of HCV IRES-mediated translation initiation. Cell. 2004 Oct 29;119(3):369-80. doi: 10.1016/j.cell.2004.09.038. PMID: 15507208.

Blanchard SC, Gonzalez RL, Kim HD, Chu S, Puglisi JD. tRNA selection and kinetic proofreading in translation. Nat Struct Mol Biol. 2004 Oct;11(10):1008-14. doi: 10.1038/nsmb831. Epub 2004 Sep 26. PMID: 15448679.

Blanchard SC, Kim HD, Gonzalez RL Jr, Puglisi JD, Chu S. tRNA dynamics on the ribosome during translation. Proc Natl Acad Sci U S A. 2004 Aug 31;101(35):12893-8. doi: 10.1073/pnas.0403884101. Epub 2004 Aug 18. PMID: 15317937.

Lukavsky PJ, Puglisi JD. Large-scale preparation and purification of polyacrylamide-free RNA oligonucleotides. RNA. 2004 May;10(5):889-93. doi: 10.1261/rna.5264804. PMID: 15100443.

2003

Runyon ST, Puglisi JD. Design of a cyclic peptide that targets a viral RNA. J Am Chem Soc. 2003 Dec 24;125(51):15704-5. doi: 10.1021/ja036344h. PMID: 14677935.

Schroeder SJ, Fountain MA, Kennedy SD, Lukavsky PJ, Puglisi JD, Krugh TR, Turner DH. Thermodynamic stability and structural features of the J4/5 loop in a Pneumocystis carinii group I intron. Biochemistry. 2003 Dec 9;42(48):14184-96. doi: 10.1021/bi0301587. PMID: 14640686.

Lukavsky PJ, Kim I, Otto GA, Puglisi JD. Structure of HCV IRES domain II determined by NMR. Nat Struct Biol. 2003 Dec;10(12):1033-8. doi: 10.1038/nsb1004. Epub 2003 Oct 26. PMID: 14578934.

Puglisi JD, Kim I, Lukavsky P, Otto G, Lancaster A, Sarnow P. Structural and functional investigation of the hepatitis C virus IRES. Nucleic Acids Res Suppl. 2001;(1):263. doi: 10.1093/nass/1.1.263. PMID: 12836365.

Li Q, Khosla C, Puglisi JD, Liu CW. Solution structure and backbone dynamics of the holo form of the frenolicin acyl carrier protein. Biochemistry. 2003 Apr 29;42(16):4648-57. doi: 10.1021/bi0274120. PMID: 12705828.

Lynch SR, Gonzalez RL, Puglisi JD. Comparison of X-ray crystal structure of the 30S subunit-antibiotic complex with NMR structure of decoding site oligonucleotide-paromomycin complex. Structure. 2003 Jan;11(1):43-53. doi: 10.1016/s0969-2126(02)00934-6. PMID: 12517339.

2002

Otto GA, Lukavsky PJ, Lancaster AM, Sarnow P, Puglisi JD. Ribosomal proteins mediate the hepatitis C virus IRES-HeLa 40S interaction. RNA. 2002 Jul;8(7):913-23. doi: 10.1017/s1355838202022057. PMID: 12166646.

Kim I, Lukavsky PJ, Puglisi JD. NMR study of 100 kDa HCV IRES RNA using segmental isotope labeling. J Am Chem Soc. 2002 Aug 14;124(32):9338-9. doi: 10.1021/ja026647w. PMID: 12167005.

Yoshizawa S, Fourmy D, Eason RG, Puglisi JD. Sequence-specific recognition of the major groove of RNA by deoxystreptamine. Biochemistry. 2002 May 21;41(20):6263-70. doi: 10.1021/bi0121609. PMID: 12009887.

Lukavsky PJ, Puglisi JD. RNAPack: an integrated NMR approach to RNA structure determination. Methods. 2001 Nov;25(3):316-32. doi: 10.1006/meth.2001.1244. PMID: 11860286.

2001

Recht MI, Puglisi JD. Aminoglycoside resistance with homogeneous and heterogeneous populations of antibiotic-resistant ribosomes. Antimicrob Agents Chemother. 2001 Sep;45(9):2414-9. doi: 10.1128/AAC.45.9.2414-2419.2001. PMID: 11502507.

Blanchard SC, Puglisi JD. Solution structure of the A loop of 23S ribosomal RNA. Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3720-5. doi: 10.1073/pnas.051608498. Epub 2001 Mar 20. PMID: 11259644.

Lynch SR, Puglisi JD. Structure of a eukaryotic decoding region A-site RNA. J Mol Biol. 2001 Mar 9;306(5):1023-35. doi: 10.1006/jmbi.2000.4419. PMID: 11237616.

Lynch SR, Puglisi JD. Structural origins of aminoglycoside specificity for prokaryotic ribosomes. J Mol Biol. 2001 Mar 9;306(5):1037-58. doi: 10.1006/jmbi.2000.4420. PMID: 11237617.

2000

Lukavsky PJ, Otto GA, Lancaster AM, Sarnow P, Puglisi JD. Structures of two RNA domains essential for hepatitis C virus internal ribosome entry site function. Nat Struct Biol. 2000 Dec;7(12):1105-10. doi: 10.1038/81951. PMID: 11101890.

Puglisi JD, Blanchard SC, Green R. Approaching translation at atomic resolution. Nat Struct Biol. 2000 Oct;7(10):855-61. doi: 10.1038/79603. PMID: 11017192.

Dahlquist KD, Puglisi JD. Interaction of translation initiation factor IF1 with the E. coli ribosomal A site. J Mol Biol. 2000 May 26;299(1):1-15. doi: 10.1006/jmbi.2000.3672. PMID: 10860719.

Lynch SR, Recht MI, Puglisi JD. Biochemical and nuclear magnetic resonance studies of aminoglycoside-RNA complexes. Methods Enzymol. 2000;317:240-61. doi: 10.1016/s0076-6879(00)17018-1. PMID: 10829284.

Puglisi JD. mRNA processing: the 3′-end justifies the means. Nat Struct Biol. 2000 Apr;7(4):263-4. doi: 10.1038/74008. PMID: 10742163.

1999

Green R, Puglisi JD. The ribosome revealed. Nat Struct Biol. 1999 Nov;6(11):999-1003. doi: 10.1038/14881. PMID: 10542087.

Yoshizawa S, Fourmy D, Puglisi JD. Recognition of the codon-anticodon helix by ribosomal RNA. Science. 1999 Sep 10;285(5434):1722-5. doi: 10.1126/science.285.5434.1722. PMID: 10481006.

Recht MI, Douthwaite S, Puglisi JD. Basis for prokaryotic specificity of action of aminoglycoside antibiotics. EMBO J. 1999 Jun 1;18(11):3133-8. doi: 10.1093/emboj/18.11.3133. PMID: 10357824.

Recht MI, Douthwaite S, Dahlquist KD, Puglisi JD. Effect of mutations in the A site of 16 S rRNA on aminoglycoside antibiotic-ribosome interaction. J Mol Biol. 1999 Feb 12;286(1):33-43. doi: 10.1006/jmbi.1998.2446. PMID: 9931247.

1998

Puglisi EV, Puglisi JD. HIV-1 A-rich RNA loop mimics the tRNA anticodon structure. Nat Struct Biol. 1998 Dec;5(12):1033-6. doi: 10.1038/4141. PMID: 9846871.

Yoshizawa S, Fourmy D, Puglisi JD. Structural origins of gentamicin antibiotic action. EMBO J. 1998 Nov 16;17(22):6437-48. doi: 10.1093/emboj/17.22.6437. PMID: 9822590.

Blanchard SC, Fourmy D, Eason RG, Puglisi JD. rRNA chemical groups required for aminoglycoside binding. Biochemistry. 1998 May 26;37(21):7716-24. doi: 10.1021/bi973125y. PMID: 9601031.

Fourmy D, Yoshizawa S, Puglisi JD. Paromomycin binding induces a local conformational change in the A-site of 16 S rRNA. J Mol Biol. 1998 Mar 27;277(2):333-45. doi: 10.1006/jmbi.1997.1551. PMID: 9514734.

Fourmy D, Recht MI, Puglisi JD. Binding of neomycin-class aminoglycoside antibiotics to the A-site of 16 S rRNA. J Mol Biol. 1998 Mar 27;277(2):347-62. doi: 10.1006/jmbi.1997.1552. PMID: 9514735.

1997

Puglisi EV, Green R, Noller HF, Puglisi JD. Structure of a conserved RNA component of the peptidyl transferase centre. Nat Struct Biol. 1997 Oct;4(10):775-8. doi: 10.1038/nsb1097-775. PMID: 9334738.

1996

Fourmy D, Recht MI, Blanchard SC, Puglisi JD. Structure of the A site of Escherichia coli 16S ribosomal RNA complexed with an aminoglycoside antibiotic. Science. 1996 Nov 22;274(5291):1367-71. doi: 10.1126/science.274.5291.1367. PMID: 8910275.

Recht MI, Fourmy D, Blanchard SC, Dahlquist KD, Puglisi JD. RNA sequence determinants for aminoglycoside binding to an A-site rRNA model oligonucleotide. J Mol Biol. 1996 Oct 4;262(4):421-36. doi: 10.1006/jmbi.1996.0526. PMID: 8893854.

1995

Puglisi JD, Chen L, Blanchard S, Frankel AD. Solution structure of a bovine immunodeficiency virus Tat-TAR peptide-RNA complex. Science. 1995 Nov 17;270(5239):1200-3. doi: 10.1126/science.270.5239.1200. PMID: 7502045.

Puglisi JD, Wyatt JR. Biochemical and NMR studies of RNA conformation with an emphasis on RNA pseudoknots. Methods Enzymol. 1995;261:323-50. doi: 10.1016/s0076-6879(95)61016-2. PMID: 8569502.

Dahlquist K, Puglisi JD. Investigating the structure and function of translation initiation factor 1 in Escherichia coli. Nucleic Acids Symp Ser. 1995;(33):170-1. PMID: 8643361.

1994

Puglisi EV, Puglisi JD, Williamson JR, RajBhandary UL. NMR analysis of tRNA acceptor stem microhelices: discriminator base change affects tRNA conformation at the 3′ end. Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11467-71. doi: 10.1073/pnas.91.24.11467. PMID: 7972085.

1993

Pütz J, Puglisi JD, Florentz C, Giegé R. Additive, cooperative and anti-cooperative effects between identity nucleotides of a tRNA. EMBO J. 1993 Jul;12(7):2949-57. PMID: 8335008.

Puglisi JD, Chen L, Frankel AD, Williamson JR. Role of RNA structure in arginine recognition of TAR RNA. Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3680-4. doi: 10.1073/pnas.90.8.3680. PMID: 7682716.

Caruso A, Tiberio L, De Rango C, Bonfanti C, Flamminio G, Gribaudo G, Monti E, Viani E, Manca N, Garotta G, et al. A monoclonal antibody to the NH2-terminal segment of human IFN-gamma selectively interferes with the antiproliferative activity of the lymphokine. J Immunol. 1993 Feb 1;150(3):1029-35. PMID: 8423329.

Puglisi JD, Pütz J, Florentz C, Giegé R. Influence of tRNA tertiary structure and stability on aminoacylation by yeast aspartyl-tRNA synthetase. Nucleic Acids Res. 1993 Jan 11;21(1):41-9. doi: 10.1093/nar/21.1.41. PMID: 8441619.

Giegé R, Puglisi JD, Florentz C. tRNA structure and aminoacylation efficiency. Prog Nucleic Acid Res Mol Biol. 1993;45:129-206. doi: 10.1016/s0079-6603(08)60869-7. PMID: 8341800.

1992

Batey RT, Inada M, Kujawinski E, Puglisi JD, Williamson JR. Preparation of isotopically labeled ribonucleotides for multidimensional NMR spectroscopy of RNA. Nucleic Acids Res. 1992 Sep 11;20(17):4515-23. doi: 10.1093/nar/20.17.4515. PMID: 1383928.

Perret V, Florentz C, Puglisi JD, Giegé R. Effect of conformational features on the aminoacylation of tRNAs and consequences on the permutation of tRNA specificities. J Mol Biol. 1992 Jul 20;226(2):323-33. doi: 10.1016/0022-2836(92)90950-o. PMID: 1640453.

Puglisi JD, Tan R, Calnan BJ, Frankel AD, Williamson JR. Conformation of the TAR RNA-arginine complex by NMR spectroscopy. Science. 1992 Jul 3;257(5066):76-80. doi: 10.1126/science.1621097. PMID: 1621097.

Rudinger J, Puglisi JD, Pütz J, Schatz D, Eckstein F, Florentz C, Giegé R. Determinant nucleotides of yeast tRNA(Asp) interact directly with aspartyl-tRNA synthetase. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5882-6. doi: 10.1073/pnas.89.13.5882. PMID: 1631068.

Turano A, Balsari A, Viani E, Landolfo S, Zanoni L, Gargiulo F, Caruso A. Natural human antibodies to gamma interferon interfere with the immunomodulating activity of the lymphokine. Proc Natl Acad Sci U S A. 1992 May 15;89(10):4447-51. doi: 10.1073/pnas.89.10.4447. PMID: 1316609.

Caruso A, Viani E, Tiberio L, Pollara P, Monti E, Bonfanti C, Gao J, Landolfo S, Balsari A, Turano A. Inhibition of the biological activity of human interferon-gamma by antipeptide antibodies. J Interferon Res. 1992 Feb;12(1):49-54. doi: 10.1089/jir.1992.12.49. PMID: 1573282.

1991

Wyatt JR, Chastain M, Puglisi JD. Synthesis and purification of large amounts of RNA oligonucleotides. Biotechniques. 1991 Dec;11(6):764-9. PMID: 1809333.

Viani E, Flamminio G, Caruso A, Foresti I, De Francesco M, Pollara P, Balsari A, Turano A. Purification of natural human IFN-gamma antibodies. Immunol Lett. 1991 Sep;30(1):53-8. doi: 10.1016/0165-2478(91)90089-s. PMID: 1959942.

Pütz J, Puglisi JD, Florentz C, Giegé R. Identity elements for specific aminoacylation of yeast tRNA(Asp) by cognate aspartyl-tRNA synthetase. Science. 1991 Jun 21;252(5013):1696-9. doi: 10.1126/science.2047878. PMID: 2047878.

1990

Puglisi JD, Wyatt JR, Tinoco I Jr. Conformation of an RNA pseudoknot. J Mol Biol. 1990 Jul 20;214(2):437-53. doi: 10.1016/0022-2836(90)90192-O. PMID: 1696318.

Wyatt JR, Puglisi JD, Tinoco I Jr. RNA pseudoknots. Stability and loop size requirements. J Mol Biol. 1990 Jul 20;214(2):455-70. doi: 10.1016/0022-2836(90)90193-P. PMID: 1696319.

Puglisi JD, Wyatt JR, Tinoco I Jr. Solution conformation of an RNA hairpin loop. Biochemistry. 1990 May 1;29(17):4215-26. doi: 10.1021/bi00469a026. PMID: 1694459.

1989

Wyatt JR, Puglisi JD, Tinoco I Jr. RNA folding: pseudoknots, loops and bulges. Bioessays. 1989 Oct;11(4):100-6. doi: 10.1002/bies.950110406. PMID: 2695075.

Puglisi JD, Tinoco I Jr. Absorbance melting curves of RNA. Methods Enzymol. 1989;180:304-25. doi: 10.1016/0076-6879(89)80108-9. PMID: 2482421.

1988

Puglisi JD, Wyatt JR, Tinoco I Jr. A pseudoknotted RNA oligonucleotide. Nature. 1988 Jan 21;331(6153):283-6. doi: 10.1038/331283a0. PMID: 3336440.

1987

Trulson MO, Cruz P, Puglisi JD, Tinoco I Jr, Mathies RA. Raman spectroscopic study of left-handed Z-RNA. Biochemistry. 1987 Dec 29;26(26):8624-30. doi: 10.1021/bi00400a020. PMID: 2450564.

Hardin CC, Zarling DA, Puglisi JD, Trulson MO, Davis PW, Tinoco I Jr. Stabilization of Z-RNA by chemical bromination and its recognition by anti-Z-DNA antibodies. Biochemistry. 1987 Aug 11;26(16):5191-9. doi: 10.1021/bi00390a044. PMID: 2444254.

Tinoco I Jr, Davis PW, Hardin CC, Puglisi JD, Walker GT, Wyatt J. RNA structure from A to Z. Cold Spring Harb Symp Quant Biol. 1987;52:135-46. doi: 10.1101/sqb.1987.052.01.018. PMID: 2456875.